In his article on radical innovation, Shinn (2005) examined the role of scientific instruments in innovation. This paper continues to investigate this theme, but the main focus is on how scientists or engineers from one discipline may learn from another and produce new knowledge and new technology. The paper looks at the role that tools and instruments developed by one discipline, in one environment, can play in the development of knowledge in a new environment. The theoretical basis for this study (...) is Vygotsky’s (1978) concept of tool-mediated activity. The proposed conceptualisation views instruments as dynamic and suggests types of tool-mediated activities which may contribute to knowledge creation. The collaborative process of experimentation is examined and opportunities for knowledge creation are discussed in relation to the instruments used. Methods used are interviews and observations. The case study is a small multidisciplinary laboratory developing a new process for producing nanoreactors, with potential applications in pharmaceuticals and energy. (shrink)